Automatic quantification and topographical distribution of the whole population of S and L cones in the adult albino and pigmented rats.
|Autores:||Ortin-Martinez A, Jimenez-Lopez M, Nadal-Nicolas FM, Salinas-Navarro M, Alarcon-Martinez L, Sauvé Y, Villegas-Perez MP, Vidal-Sanz M, Agudo-Barriuso M|
|Títuto Revista:||Invest Ophthalmol Vis Sci.|
Purpose: To quantify the whole population of S and L cones in the albino (Sprague Dawley, SD) and pigmented (Pieblad Virol Glax, PVG) rats and to study their topographical distribution within the retina Methods: Retinal radial sections and whole mounted retinas were double immunodetected with antibodies against UV-sensitive and L opsins to detect the S and L cones, respectively. Two automatic routines were developed to quantify the whole population of S and L cones. Detailed isodensity maps of each cone type were generated. In both strains, it was detected the presence of dual cones, these were semi-automatically quantified and their distribution determined. Matching distribution of retinal ganglion cells (RGC) and L cones was attained by double immunodetection of Brn3a and L Opsin, respectively Results: The mean number+/-SEM of L or S cones in SD and PVG retinas is 231,736+/-14,517 and 239,939+/-6,494 or 41,028+/-5,074 and 27,316+/- 2,235, respectively. There is an increasing gradient of the S cones density along the infero-nasal quadrant, though their highest densities are found in the retinal rims. The distribution of L cones seems to be complementary to the S cones. Their highest densities are observed in the superior naso-temporal axis, paralleling the distribution of Brn3a positive RGCs. Conclusions: These data establish, for the first time, the total number and the topographical distribution of S and L cones in two rat strains and demonstrate the correlation of L cones and RGC spatial distribution.