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"Super p53" Mice Display Retinal Astroglial Changes.

Tipo: Artículo
Código: PMID: 23762373
Autores: Salazar JJ, Gallego-Pinazo R, de Hoz R, Pinazo-Durán MD, Rojas B, Ramírez AI, Serrano M, Ramírez JM.
Títuto Revista: PLoS One.
Colaboradores: RD12-0034-0002_Instituto de Investigaciones Oftalmológicas "Ramón Castroviejo", Universidad Complutense de Madrid; RD12-0034-0008_Hospital Universitario y Politécnico La Fe de Valencia.
Centro: Hospital Clínico San Carlos (HCSC)

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Abstract

Tumour-suppressor genes, such as the p53 gene, produce proteins that inhibit cell division under adverse conditions, as in the case of DNA damage, radiation, hypoxia, or oxidative stress (OS). The p53 gene can arrest proliferation and trigger death by apoptosis subsequent to several factors. In astrocytes, p53 promotes cell-cycle arrest and is involved in oxidative stress-mediated astrocyte cell death. Increasingly, astrocytic p53 is proving fundamental in orchestrating neurodegenerative disease pathogenesis. In terms of ocular disease, p53 may play a role in hypoxia due to ischaemia and may be involved in the retinal response to oxidative stress (OS). We studied the influence of the p53 gene in the structural and quantitative characteristics of astrocytes in the retina. Adult mice of the C57BL/6 strain (12 months old) were distributed into two groups: 1) mice with two extra copies of p53 ("super p53"; n?=?6) and 2) wild-type p53 age-matched control, as the control group (WT; n?=?6). Retinas from each group were immunohistochemically processed to locate the glial fibrillary acidic protein (GFAP). GFAP+ astrocytes were manually counted and the mean area occupied for one astrocyte was quantified. Retinal-astrocyte distribution followed established patterns; however, morphological changes were seen through the retinas in relation to p53 availability. The mean GFAP+ area occupied by one astrocyte in "super p53" eyes was significantly higher (p<0.05; Student's t-test) than in the WT. In addition, astroglial density was significantly higher in the "super p53" retinas than in the WT ones, both in the whole-retina (p<0,01 Student's t-test) and in the intermediate and peripheral concentric areas of the retina (p