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Antioxidant N-acetyl-cysteine protects retinal pigmented epithelial cells from long-term hypoxia changes in gene expression.

Tipo: Artículo
Autores: Gerona G, López D, Palmero M, Maneu V.
Títuto Revista: Journal of Ocular Pharmacology and Therapeutics
Referencia:
Centro: 13 - UA
J Ocul Pharmacol Ther. 2010 Aug;26(4):309-14.

Antioxidant N-acetyl-cysteine protects retinal pigmented epithelial cells from long-term hypoxia changes in gene expression.

Source

Departamento de Optica, Farmacología y Anatomía, Universidad de Alicante , San Vicente del Raspeig, Spain.

Abstract

PURPOSE:

To further know the signaling pathways involved in hypoxia-induced apoptosis in retinal pigmented epithelial (RPE) cells and to improve the understanding of the antioxidant N-acetyl-cysteine (NAC) treatment effect.

METHODS:

We analyzed the expression levels of several apoptosis-related genes by semiquantitative reverse transcriptase-polymerase chain reaction in RPE after 72 h of maintained hypoxia, with or without 10 mM NAC treatment.

RESULTS:

Under hypoxic conditions, we detected a higher expression level of p53 and CASP8. Cell treatment with NAC 10 mM prevented this increase. Other apoptosis-related genes such as bax, CASP3, CASP4, CASP7, and fas did not show an increase in expression levels in hypoxia.

CONCLUSIONS:

NAC prevents the increased expression levels of p53 and CASP8 induced by long-term maintained hypoxia. The supply of antioxidants could be a useful preventive approach in protecting RPE from the effects of chronic oxygen stress, which is of great interest in oxygen stress-related diseases such as age-related macular degeneration and other senescence-associated pathologies.

PMID: 20653475 [PubMed - indexed for MEDLINE]